N_N20R1 Cell Line
Exclusive toolThe p53 mediated responses of the parental and resistant cell line were compared. In contrast to the parental cell lines, p53 activation by Nutlin-3, MI-63 or ionizing radiation was not observed in the NGP derived cell line, N_N20R1.
Contributors
| Inventor | Institute |
|---|---|
| John Lunec | Newcastle University |
| SKU: | 152840 |
|---|---|
| Product description: | To determine how resistance to MDM2/p53 binding antagonists might develop, NGP cells were exposed to growth inhibitory concentrations of a MDM2 inhibitor, Nutlin-3, and a clonal resistant cell line was generated. The p53 mediated responses of the parental and resistant cell line were compared. In contrast to the parental cell lines, p53 activation by Nutlin-3, MI-63 or ionizing radiation was not observed in the NGP derived cell line, N_N20R1. |
| Conditional: | No |
| Production details: | Resistant cell lines were established by exposing NGP cells to Nutlin-3. Single cell derived colonies were isolated with cloning cylinders and the clonal population expanded in culture medium containing the MDM2/p53 antagonist refreshed weekly for 60 days. Stage 1 resistant clones were then further exposed to increased concentrations of Nutlin-3 for 30 days to generate stage 2 resistant clones. |
| Cellosaurus ID: | CVCL_HG08 |
| Parental cell line: | NGP |
| Disease: | Cancer |
| Cat. #: | 152840 |
|---|---|
| Provenance and Ownership: | The N_N20R1 Cell Line is owned by Cancer Research UK. CancerTools supplies the N_N20R1 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing N_N20R1 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Neuroblastoma |
| Organism: | Human |
| Tissue: | Lung |
| Model: | Cancer Model |
| Cancer Types In Detail: | Neuroblastoma |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation. Drummond et al. 2016. Oncotarget. :. PMID: 27323823. |
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N_N20R1 Cell Line. Early log phase.
N_N20R1 Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
