MM057 cell line
Eumelanotic cell line derived from a lymph node metastasis. This cell line displays an intermediate phenotype (melanocytic state with increased mesenchymal-like properties) . MM057 displays an intermediate sensitivity to MEKi
Contributors
| Inventor | Institute |
|---|---|
| Ghanem Elias, Ahmad Najem | Universit? libre de Bruxelles |
| SKU: | 161621 |
|---|---|
| Product description: | Eumelanotic cell line derived from a lymph node metastasis. This cell line displays an intermediate phenotype (melanocytic state with increased mesenchymal-like properties) . MM057 displays an intermediate sensitivity to MEKi |
| Gender: | Female |
| CRISPR: | No |
| Parental cell line: | Ham’s F10 melanoma culture medium (HMCM) |
| Model description: | This cell line displays an intermediate phenotype ( melanocytic state with increased mesenchymal-like properties) |
| Disease: | Cancer |
| Cat. #: | 161621 |
|---|---|
| Cancer types: | Skin cancer |
| Research Fields: | Cancer |
| Organism: | Human |
| Tissue: | Lymphatic |
| Gender: | Female |
| Model: | NRAS: Q61R, MET: N375S |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Melanoma |
| Primary citation: | Gembarska et al. 2012. Nat Med 18, 12391247. PMID: 22820643 |
| Initial handling information: | The frozen culture should be stored in liquid nitrogen upon arrival or thaw the vial and initiate the culture as soon as possible upon receipt |
|---|---|
| Format: | Frozen |
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry Ice |
| Growth medium: | Hamâs F10 supplemented with 10% of fetal calf serum (FCS), with L-glutamine (1mM), penicillin (100 IU/ml), and streptomycin (100 Â?g/ml). Renewal 2 to 3 times per week |
| Subculture routine: | Split sub-confluent cultures (70-80%) 1:2 to 1:5 |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| Biosafety level: | 2 |
| References: |
Sabbah et al. 2021. Mol Cancer Res. 19(7):1221-1233. PMID: 33741716 Krayem et al. 2014. Eur J Cancer. 50(7):1310-20. PMID: 24559688 Krayem et al. 2018. Oncotarget. 9(61):31888-31903. PMID: 30159130 Soumoy et al. 2020. Cancers (Basel). 12(5):1323. PMID: 32455924 Porcelli et al. 2015. J Transl Med. 13:26. PMID: 25623468 Wouterset al. 2020. Nat Cell Biol. 22(8):986-998. PMID: 32753671 Verfaillie et al. 2015. Nat Commun 6, 6683. PMID: 25865119 Aftimos et al. 2013. Int J Oncol. 43(3)… |
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