immortal melanocyte cell lines derived from the skin of neonatal Tyrp1b/Tyrp1b Ink4aArf -/- C57BL/6J mice
| Inventor | Institute |
|---|---|
| Elena Sviderskaya | St. George's University London |
| SKU: | 162005 |
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| Product description: | The melan-Ink4a-Arf-b1, -b2 and -b3 cell lines are immortal melanocyte cell lines derived from the skin of neonatal Tyrp1b/Tyrp1b Ink4aArf -/- C57BL/6J mice. These melanocytes do not express the melanogenic enzyme tyrosinase-related protein 1 (Tyrp1), or p16Ink4A and Arf. These melanocytes display brown pigmentation in culture which is reduced compared to cell lines that have functional Tyrp1 but are derived from Ink4aArf -/- C57BL/6J mice (such as melan-Ink4a-Arf-1,-2, -3 and -4 which are also available from CancerTools.org. These melanocytes can be used as a model of Tyrp1-null skin melanocytes in studies focussing on skin cancers and melanomas. They are also a useful tool for studies of pigmentation genetics. |
| Cat. #: | 162005 |
|---|---|
| Organism: | Mouse |
| Tissue: | Skin |
| Model: | Immortalised non-cancerous cell lines |
| Morphology: | Small, oval-fusiform |
| Primary citation: | Sviderskaya EV, et al. J Natl Cancer Inst. 2002 Mar 20;94(6):446-54. PMID: 11904317 |
| Storage conditions: | liquid N2 |
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| Shipping conditions: | Dry Ice |
| Growth medium: | RPMI + FBS (10%) + TPA (200 nM) + cholera toxin (CT) (200 pM) |
| References: |
Sviderskaya EV, et al. J Natl Cancer Inst. 2002 Mar 20 94(6):446-54. PMID: 11904317 |
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