MDA MB-231 sgRNA1 & 2 cell line

The MDA MB-231 sgRNA1 & 2 cell line contains site-specific methylations in the BRCA1 promoter, analogous to the sites of methylation in breast cancer patients.

Contributors

Inventor Institute
Priya Srinivas Rajiv Gandhi Centre for Biotechnology (RGCB)
SKU: 161601
Product description: MDA-MB-231 sgRNA1 & 2 cell line ÂÂ
Gender: Female
CRISPR: Yes
Conditional: Yes
Conditional description: conditional BRCA1 Hypermethylated cell line which gets reverted in the presence of tetracycline/doxycycline
Production details: Cells with wildtype unmethylated BRCA1 background were infected with lentiviruses that encode for deadCas9- DNMT3A fusion protein and synthetic guide RNAs under the control of a tet-off promoter. After successful infection, the cells were then established following FACS sorting.
Additional notes: 1?g/ml of doxycycline for 12 hours can stop the eGFP signal from pCL-CTIG and BRCA1 hypermethylation will be reverted.
Receptors of note: Expression of BRCA1 only in the presence of doxycycline
Parental cell line: MDA MB 234
Model description: BRCA1 hypermethylated cells developed using CRISPR-Cas9 technology
Disease: Cancer
Cat. #: 161601
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer
Organism: Human
Tissue: Breast
Gender: Female
Model: Eipgenetic modification
Morphology: Epithelial slightly elongated morphology
Growth properties: Adherent
Cancer Types In Detail: Breast carcinoma
Primary citation: https://doi.org/10.1101/2022.04.30.490089
Initial handling information: Store in Liquid Nitrogen. Thaw in 10% DMEM with 1% Penicillin-Streptomycin
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: DMEM medium supplemented with 10% FBS and 1% Penicillin-Streptomycin
Subculture routine: Split the cells into 1:3 ratio when the cells reach 80% Confluency
Temperature: 37° C
Atmosphere: 5% CO2
Storage medium: 90% FBS + 1% Penicillin-Streptomycin + 10% sterile DMSO
Recommended controls: MDA-MB-231 sgRNA Control cell line, MCF-7 sgRNA1 controls Control cell line , MCF-7 sgRNA2 Control cell line
Biosafety level: 2
References:

https://doi.org/10.1101/2022.04.30.490089

Related Tools for Cell lines

Cat. # Tool Name
151579 U-2 OS Gal4-p300 Cell Line Key Info View Tool
151558 MEF Trex1 KO Cell Line Key Info View Tool
151425 TR146 Cell Line Key Info View Tool
151488 B6.1.1 Mouse ES Cell Line Key Info View Tool
151663 MEF PKCe KO KI Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.