MDA-MB-231 CAGA12-Luc/TK Renilla cell line

The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for Activin and TGF-beta signalling.

Contributors

Inventor Institute
Caroline Hill The Francis Crick Institute
Cat. #: 156437
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Cell signaling and signal transduction;Developmental biology
Organism: Human
Tissue: Breast
Model: Reporter
Cancer Types In Detail: Breast cancer
Product description: The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for Activin and TGF-beta signalling. The cell line stably expresses CAGA12-Luc, a Smad3/Smad4-dependent reporter containing 12 copies of the CAGAC sites (AGAC motif underlined) from the PAI-1 promoter. This is a Smad3/Smad4-dependent reporter, although it also binds complexes of Smad2 exon 3 with Smad4. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.
Conditional description: In the presence of activin and TGF-beta signalling luciferase is expressed.
Production details: Made by transfecting the breast cancer cell line MDA-MB-231 with CAGA12-Luc with pRetroSuper for puromycin resistance and then TK Renilla with pCMV-Bsd for blasticidin resistance.Clonal
Parental cell line: MDA-MB-231 cell line
Disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)+ 50 ?g/ml blasticidin
Recommended controls: MDA-MB-231 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Grnroos et al. 2012. Mol Cell Biol. 32(14):2904-16. PMID: 22615489.

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