MCF7aro Let-R Cell Line is a human cell line model of aromatase inhibitor resistant breast cancer.
| Inventor | Institute |
|---|---|
| Marie McIlroy | Royal College of Surgeons Ireland (RCSI) |
| SKU: | 151781 |
|---|---|
| Product description: | MCF7aro Let-R Cell Line is a human cell line model of aromatase inhibitor resistant breast cancer. |
| Conditional: | Yes |
| Production details: | Letrozole (aromatase inhibitor) sensitive cells (MCF-7aro) were developed by stable transfection of the aromatase gene (CYP19); Letrozole-resistant cells (MCF-7aro Let-R) were created by long-term treatment of aromatase-expressing MCF7 cells (MCF7aro) with letrozole. |
| Cellosaurus ID: | CVCL_W348 |
| Parental cell line: | MCF7 |
| Disease: | Cancer |
| Cat. #: | 151781 |
|---|---|
| Provenance and Ownership: | The MCF7aro Let-R Cell Line is owned by Cancer Research UK. CancerTools supplies the MCF7aro Let-R Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing MCF7aro Let-R Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Breast cancer |
| Research Fields: | Cancer; Drug development |
| Organism: | Human |
| Tissue: | Breast |
| Model: | Tumour line |
| Cancer Types In Detail: | Breast cancer |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | They are cultured in phenol red free Eagle’s Minimum Essential Medium (Phenol-red free MEM) (Sigma Aldrich), 10% charcoal dextran stripped FCS (CDS-FCS), 1% Pen-Strep (Sigma Aldrich), 2 mM L-Glutamine (Sigma Aldrich) and 200 ??g/ml G418 disulfate salt (Geneticin) (Sigma Aldrich), 2.5-8 M androstenedione and 10-6 M letrozole. |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Cellosaurus MCF-7aro Let-R (CVCL_W348) O'Hara et al. 2012. Clin Cancer Res. 18(12):3305-15. PMID: 22550166. AIB1:ERa transcriptional activity is selectively enhanced in aromatase inhibitor-resistant breast cancer cells. McIlroy et al. 2010. Cancer Res. 70(4):1585-94. PMID: 20145129. Interaction of developmental transcription factor HOXC11 with steroid receptor coactivator SRC-1 mediates resistance to endocrine therapy in breast cancer [corrected]. |
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