MCF7 rho-0 cell line lacking mitochondrial DNA and oxidative phosphorylation
| Inventor | Institute |
|---|---|
| Mikhail Alexeyev | University of South Alabama |
| SKU: | 162462 |
|---|---|
| Product description: | MCF7 rho-0 cell line lacking mitochondrial DNA and oxidative phosphorylation |
| Gender: | Female |
| CRISPR: | No |
| Conditional: | No |
| Production details: | These cells were derived from the parental MCF7 cell line by prolonged (69 days) treatment with ethidium bromide, an inhibitor of mtDNA replication. The resulting cell line retains the STR profile of the original parental cell line. However, it is unable to conduct oxidative phosphorylation (OXPHOS). |
| Receptors of note: | Estrogen and progesterone receptor positive, HER2 negative. |
| Parental cell line: | MCF7 |
| Disease: | Cancer |
| Cat. #: | 162462 |
|---|---|
| Cancer types: | Breast cancer |
| Organism: | Human |
| Tissue: | Breast |
| Gender: | Female |
| Morphology: | Epithelial |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Adenocarcinoma |
| Initial handling information: | Upon arrival, store in liquid nitrogen or at -80°C (for up to two weeks). Thaw in a 37°C water bath, mix with 10 ml of fresh growth medium, spin down at 300g, resuspend in 10 ml of fresh medium, and transfer into 100 mm TC treated culture plate, which should be incubated at 37°C with 5% CO2, humidified. |
|---|---|
| Format: | Frozen |
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | High-glucose DMEM (4.5 g/l glucose) supplemented with 20% fetal bovine serum (FBS), 1 mM sodium pyruvate, 50 μg/mL uridine, 50 μg/mL gentamycin |
| Subculture routine: | Trypsinize with 0.05% trypsin for 5-10 min at 37°C, neutralize trypsin with fresh medium and split 1:3 every 7-14 days. |
| Temperature: | 37°C |
| Atmosphere: | 5% CO2 |
| Recommended controls: | Wild type MCF7 cells |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
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