MCF7/164R-4 Cell Line

The MCF7/164R-4 cell line is a breast cancer cell line resistant to fulvestrant (Faslodex). The MCF7/164R-4 cell line is a human breast cancer cell line established from MCF7. The cellular classification is epithelial, and their shape is polygonal. The passage number of this cell line is 403 (AL3810, AL3811). Treatment with the steroidal antiestrogen fulvestrant has proven effective upon progression on tamoxifen therapy and is now approved for second-line treatment after tamoxifen or aromatase inhibitors. As for tamoxifen treatment of advanced breast cancer, resistance will inevitably occur also for fulvestrant. Clarification of the molecular changes associated with the resistant growth…

Contributors

Inventor Institute
Anne Lykkesfeldt Danish Cancer Society, Denmark
SKU: 152101
Product description: The MCF7/164R-4 cell line is a breast cancer cell line resistant to fulvestrant (Faslodex). The MCF7/164R-4 cell line is a human breast cancer cell line established from MCF7. The cellular classification is epithelial, and their shape is polygonal. The passage number of this cell line is 403 (AL3810, AL3811). Treatment with the steroidal antiestrogen fulvestrant has proven effective upon progression on tamoxifen therapy and is now approved for second-line treatment after tamoxifen or aromatase inhibitors. As for tamoxifen treatment of advanced breast cancer, resistance will inevitably occur also for fulvestrant. Clarification of the molecular changes associated with the resistant growth is needed to find targeted treatments to resistant tumour cells and treatments that can inhibit or delay the emergence of resistance.
Alternate name: MCF-7/164R-4; 164R-4
Gender: Female
Production details: The MCF7/164R-4 cell line has been established from a clone of MCF7/S0.5 cells surviving long term growth with the pure steroidal antiestrogen ICI 164,384 in 100 nM concentration. The MCF7/164R-4 cells are also resistant to the pure steroidal antiestrogen fulvestrant (ICI 182,780) and can be maintained continuously in growth medium with 100 nM fulvestrant.
Additional notes: Upon withdrawal of fulvestrant, the cells express ER alpha, although at a reduced level compared to the parental MCF7/S0.5 cell line. The MCF7/164R-4 cells do not express progesterone receptor. The MCF7/164R-4 cells express increased level of EGFR, phosphorylated EGFR and phosphorylated ErbB3 and reduced level of ErbB4 compared to the parental MCF7/S0.5 cells.
Cellosaurus ID: CVCL_1D49
Parental cell line: MCF7 S0.5
Disease: Cancer
Cat. #: 152101
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Gender: Female
Model: Tumour line
Cancer Types In Detail: Breast cancer;Fulvestrant resistant
Primary citation: Lykkesfeldt et al. 1995. Int J Cancer. 61(4):529-534. PMID: 7759159.
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Phenol red free DMEM/F12 (1:1) supplemented with 1% FCS, Glutamax 2.5 mM and 6 ng/ml insulin. Supplemented with 100nM fulvestrant to maintain resistance.
Temperature: 37° C
Atmosphere: 5% CO2
References:

Thrane et al. 2014. Oncogene. 34(32):4199-4210. PMID: 25362855.

Sonne-Hansen et al. 2010. Breast Cancer Res Treat. 121(3):601-613. PMID: 19697122.

Frogne et al. 2008. Breast Cancer Res Treat. 114(2):263-275. PMID: 18409071.

Lykkesfeldt et al. 1995. Int J Cancer. 61(4):529-534. PMID: 7759159.

Images

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MCF7 164R-4 Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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