MCF10A hRas_V12 cell line

MCF10A cells are frequently used to study signalling pathways in breast cancer. They can be grown in 3D culture. The addition of the H-Ras12 simulates breast cancer as 50% of breast cancers display increased H-Ras activity.

Contributors

Inventor Institute
Sarah-Maria Fendt Vlaams Instituut voor Biotechnologie (VIB)
Cat. #: 156525
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer
Organism: Human
Tissue: Breast
Model: Cancer Model
Alternate name: transforming protein p21, h-Ras, HRAS
Product description: MCF10A cells are frequently used to study signalling pathways in breast cancer. They can be grown in 3D culture. The addition of the H-Ras12 simulates breast cancer as 50% of breast cancers display increased H-Ras activity.
Conditional: No
Additional notes: There is a negative control for this cell line which was made using the same pLA vector but not expressing the HRAS gene.
Parental cell line: MCF10A
Disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM-F12 supplemented with 5% horse serum, 1% penicillin (50 units ml-1), 1% streptomycin (50 ?g ml-1), 0.5 g ml-1 hydrocortisone, 100 ng ml-1 cholera toxin, 10 ?g ml-1 insulin and 20 ng ml-1 recombinant human EGF.
Mycoplasma free: Yes
Biosafety level: 1
References:

Elia et al. 2017. Nat Commun. 8:15267. PMID: 28492237.

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