MCF10A-HER2

The MCF10A-HER2 breast cancer cell line shows inducible overexpression of the HER2 protein at clinically relevant levels and can be used to study mechanisms underpinning HER2 overexpression.

Contributors

Inventor Institute
Richard Grose ; Gabriella Ficz ; Ateequllah Hayat Queen Mary University of London
Cat. #: 157986
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer
Organism: Human
Tissue: Breast
Gender: Female
Model: Immortalised Line
Cancer Types In Detail: Breast cancer
Primary citation: Hayat A. et al. 2023. Dis Model Mech. Feb 1;16(2). PMID: 36661191
Product description: The MCF10A-HER2 breast cancer cell line shows inducible overexpression of the HER2 protein at clinically relevant levels and can be used to study mechanisms underpinning HER2 overexpression. These cells also co-express GFP enabling cell tracking and selection and can be grown as 3D cultures for enhanced biological relevance. Key applications for this cell line include early molecular change detection in HER2-driven tumour development, HER2-mediated signalling pathway analysis, and HER2-targeted therapeutic screening and response profiling.
Gender: Female
Conditional: Yes
Conditional description: Conditional HER2 expression under tetracyline response element (TRE) regulated by the addition of doxycyline in breast epithelial cell line (MCF10A)
Production details: Inducible HER2 construct was stably transduced in MCF10A cell line using a third generation lentiviral transduction system.
Parental cell line: MCF10A
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Recommended controls: Any other HER2 cell line such as BT474 may be used as a positive control

Images

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MCF10A-HER2 cells cultured in 3D formed flat projecting cells of complex masses, typical of transformed cells. Adapted from Hayat A. et al. 2023. Dis Model Mech. Feb 1;16(2). PMID: 36661191.

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