MB49 Cell line
Exclusive toolGold standard murine bladder cancer model used to study checkpoint inhibitor therapies, tumour-immune interations, and preclinical immunotherapy development. Available exclusively through CancerTools. Save 30% on this cell line this autumn. Use code ACA26CELL at checkout.
Contributors
| Inventor | Institute |
|---|---|
| Leonard Franks | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| SKU: | 153368 |
|---|---|
| Product description: | MB49 is a murine bladder cancer cell line derived from C57BL/ICRF-a(t) mouse bladder epithelium transformed by chemical carcinogen exposure in culture. Originally developed by Cancer Research UK researcher Leonard Franks, MB49 is a well-established model for bladder cancer research and can be used in both in vitro studies and syngeneic in vivo mouse models. The cell line exhibits clinically relevant tumourigenic and metastatic properties, recapitulates key sex differences observed in bladder cancer, and reportedly responds to immune checkpoint inhibitors and other investigational therapies, making it a valuable model for studying disease biology and evaluating novel treatment strategies. A luminescent derivative, MB49-luc (Cat. No. 161579), is also available for in vivo imaging applications. |
| Alternate name: | MB49, MB-49, MB49 Mouse Bladder Carcinoma Cell Line |
| Gender: | Male |
| Production details: | Derived from adult C57BL/Icrf-a’ mouse bladder epithelial cells via single 24 h treatment with a chemical carcinogen on the second day of primary culture |
| Cellosaurus ID: | CVCL_7076 |
| Model description: | The cells transplanted into syngeneic mice were shown to generate carcinomas |
| Disease: | Cancer |
| Cat. #: | 153368 |
|---|---|
| Provenance and Ownership: | The MB49 Cell line is owned by Cancer Research UK. CancerTools supplies the MB49 Cell line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing MB49 Cell line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Bladder cancer |
| Research Fields: | Cancer |
| Organism: | Mouse |
| Tissue: | Bladder |
| Gender: | Male |
| Model: | Tumourigenic line |
| Morphology: | A spindle-like epithelial morphology, or rounded |
| Growth properties: | The cells do not form a 100% confluent monolayer but at 70% confluence tend to detach in small clumps that float in the media. About 10-20% of the cells will be attached with a spindle-like epithelial morphology, the remainder will appear rounded |
| Cancer Types In Detail: | Bladder carcinoma;Urinary bladder neoplasms |
| Primary citation: | Summerhayes et al. 1979. J Natl Cancer Inst. 62(4):1017-23. PMID: 107359 |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM Complete Medium or in DMEM-High Glucose with 10% FBS and 1X Penicillin/Streptomycin (optional) |
| Subculture routine: | Split sub-confluent cultures (80-85%) 1:6 to 1:10 using Accutase or trypsin-EDTA solution. Cells are cultured at 37°C, in a humidified incubator with 5% CO2. |
| Temperature: | 37° C |
| Atmosphere: | 5%CO2 |
| Storage medium: | Cells can be cryopreserved in growth medium with 10% DMSO using a Nalgene slow freeze Mr.Frosty container. |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Albertó et al. 2019. Oncol Lett. 17(3):3141-3150. PMID: 30867744. Plote et al. 2019. Oncoimmunology. 8(5):e1577125. PMID: 31069136. Shi et al. 2019. Onco Targets Ther. 12:4403-4413. PMID: 31239709. White-Gilbertson et al. 2016. Bladder (San Franc). 3(1). PMID: 26998503. Kasman et al. 2013. J Vis Exp. (82):50181. PMID: 24326612. Zhu et al. 2013. BMC Urol. 13:57. PMID: 24188098. Fabris et al. 2012. Cancer Genet. 205(4):168-176. PMID: 22559978. Chen et al. 2009. J Urol. 182(6):2932-2937. PMID: 19853870. Loskog et al. 2005. Lab Anim. 39(4):384-393. PMID: 16197705. Brocks et al. 2005. J Urol. 174(3):1115-1118. PMID: 16094076. Günther et al. 1999. Cancer Res. 59(12):2834-2837. PMID: 10383142. Summerhayes et al. 1979. J Natl Cancer Inst. 62(4):1017-1023. PMID: 107359. |
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MB49
light microscope image of MB49 in culture
Citations And Reviews
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