MB49 Cell line

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Gold standard murine bladder cancer model used to study checkpoint inhibitor therapies, tumour-immune interations, and preclinical immunotherapy development. Available exclusively through CancerTools. Save 30% on this cell line this summer. Use code SUM26CELL at checkout.

Contributors

Inventor Institute
Leonard Franks Cancer Research UK, London Research Institute: Lincoln's Inn Fields
SKU: 153368
Product description: MB49 is a murine bladder cancer cell line derived from C57BL/ICRF-a(t) mouse bladder epithelium transformed by chemical carcinogen exposure in culture. Originally developed by Cancer Research UK researcher Leonard Franks, MB49 is a well-established model for bladder cancer research and can be used in both in vitro studies and syngeneic in vivo mouse models. The cell line exhibits clinically relevant tumourigenic and metastatic properties, recapitulates key sex differences observed in bladder cancer, and reportedly responds to immune checkpoint inhibitors and other investigational therapies, making it a valuable model for studying disease biology and evaluating novel treatment strategies. A luminescent derivative, MB49-luc (Cat. No. 161579), is also available for in vivo imaging applications.
Alternate name: MB49, MB-49, MB49 Mouse Bladder Carcinoma Cell Line
Gender: Male
Production details: Derived from adult C57BL/Icrf-a’ mouse bladder epithelial cells via single 24 h treatment with a chemical carcinogen on the second day of primary culture
Cellosaurus ID: CVCL_7076
Model description: The cells transplanted into syngeneic mice were shown to generate carcinomas
Disease: Cancer
Cat. #: 153368
Provenance and Ownership: The MB49 Cell line is owned by Cancer Research UK. CancerTools supplies the MB49 Cell line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing MB49 Cell line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Bladder cancer
Research Fields: Cancer
Organism: Mouse
Tissue: Bladder
Gender: Male
Model: Tumourigenic line
Morphology: A spindle-like epithelial morphology, or rounded
Growth properties: The cells do not form a 100% confluent monolayer but at 70% confluence tend to detach in small clumps that float in the media. About 10-20% of the cells will be attached with a spindle-like epithelial morphology, the remainder will appear rounded
Cancer Types In Detail: Bladder carcinoma;Urinary bladder neoplasms
Primary citation: Summerhayes et al. 1979. J Natl Cancer Inst. 62(4):1017-23. PMID: 107359
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM Complete Medium or in DMEM-High Glucose with 10% FBS and 1X Penicillin/Streptomycin (optional)
Subculture routine: Split sub-confluent cultures (80-85%) 1:6 to 1:10 using Accutase or trypsin-EDTA solution. Cells are cultured at 37°C, in a humidified incubator with 5% CO2.
Temperature: 37° C
Atmosphere: 5%CO2
Storage medium: Cells can be cryopreserved in growth medium with 10% DMSO using a Nalgene slow freeze Mr.Frosty container.
Mycoplasma free: Yes
Biosafety level: 1
References:

Albertó et al. 2019. Oncol Lett. 17(3):3141-3150. PMID: 30867744.

Plote et al. 2019. Oncoimmunology. 8(5):e1577125. PMID: 31069136.

Shi et al. 2019. Onco Targets Ther. 12:4403-4413. PMID: 31239709.

White-Gilbertson et al. 2016. Bladder (San Franc). 3(1). PMID: 26998503.

Kasman et al. 2013. J Vis Exp. (82):50181. PMID: 24326612.

Zhu et al. 2013. BMC Urol. 13:57. PMID: 24188098.

Fabris et al. 2012. Cancer Genet. 205(4):168-176. PMID: 22559978.

Chen et al. 2009. J Urol. 182(6):2932-2937. PMID: 19853870.

Loskog et al. 2005. Lab Anim. 39(4):384-393. PMID: 16197705.

Brocks et al. 2005. J Urol. 174(3):1115-1118. PMID: 16094076.

Günther et al. 1999. Cancer Res. 59(12):2834-2837. PMID: 10383142.

Summerhayes et al. 1979. J Natl Cancer Inst. 62(4):1017-1023. PMID: 107359.

Images

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MB49 Cell Line in culture - sub confluence (A)
light microscope image of MB49 in culture

Citations And Reviews

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