KrasG12D.Map3k7−/−.GFP+ murine lung cancer cell line

A novel syngeneic KrasG12D.Map3k7-/-.GFP+ murine lung cancer cell line (E889) with orthotopic growth potential, that can serve as a useful preclinical model for evaluating tumour-host interactions, the impact of specific oncogenic alterations on the tumor microenvironment, and immunotherapeutic approaches.. This cell line was derived from the primary lung tumour of a KrasLSL-G12D/+.Map3k7fl/fl. ROSAmTmG male C57BL/6 […]

Contributors

Inventor Institute
Stephen Malkoski The University of Colorado Anschutz Medical Campus
Cat. #: 162386
Cancer types: Lung cancer
Research Fields: Cancer biology; Immunotherapy
Organism: Mouse
Tissue: Lung
Gender: Male
Primary citation: Nolan et al. 2020. Cancer Cell Int. 28: 20:417. PMID: 32874131
Alternate name: KrasG12D.Map3k7−/−.GFP+ cell line; E889 cell line
Product description: A novel syngeneic KrasG12D.Map3k7-/-.GFP+ murine lung cancer cell line (E889) with orthotopic growth potential, that can serve as a useful preclinical model for evaluating tumour-host interactions, the impact of specific oncogenic alterations on the tumor microenvironment, and immunotherapeutic approaches. This cell line was derived from the primary lung tumour of a KrasLSL-G12D/+.Map3k7fl/fl. ROSAmTmG male C57BL/6 mouse and is capable of forming orthotopic tumours in immunocompetent C57BL/6 mice. E889 cells demonstrate genetic recombination at the Kras and Map3k7 loci as shown by PCR, and express KRASG12D but not MAP3K7, as shown by western analysis. As this cell line was derived from an animal harboring the ROSAmTmG tracking allele, it also expresses GFP.
Gender: Male
Production details: An 8 week old KrasLSL-G12D/+.Map3k7fl/fl. ROSAmTmG male mouse was injected with 2 µl of 10^9 PFU/ml Ad5-CMV-Cre into the left lung. When this animal was euthanized 26 week later, an 8 mm primary tumour was passaged into a male recipient. After two passages through the flanks of recipient animals, the KrasG12D.Map3k7-/-.GFP+ cell line (E889) was established that was capable of forming orthotopic tumors in immunocompetent C57BL/6 animals.
Disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Dulbecco's Modified Eagle Medium (DMEM) containing 4.5 g/l d-glucose, l-glutamine, and sodium pyruvate, supplemented with 10% (v/v) fetal bovine serum and 100 μg/ml of primocin (optional)
Subculture routine: Split 90% confluent cultures 1:5 to 1:15 as required. Cells are cultured at 37°C, in a humidified incubator with 5% CO2.
Temperature: 37°C
Atmosphere: 5% CO2
Biosafety level: 1
References:

Nolan et al. 2020. Cancer Cell Int. 28: 20:417. PMID: 32874131

Images

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Hematoxylin and eosin stain showing orthotopic lung tumor formation by the KrasG12D.Map3k7−/−.GFP+ (E889) murine lung cancer cell line

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