KPAR1.3 Cell Line

A syngeneic murine and mutant KRAS-driven lung cancer model used to investigate tumour immunology, immune checkpoint inhibitors, and KRAS-targeted therapies. Save 30% on this cell line this summer. Use code SUM26CELL at checkout.

Contributors

Inventor Institute
Julian Downward, Jesse Boumelha, Sophie de Carne The Francis-Crick Institute
SKU: 161803
Product description: KPAR1.3 cells (also called KPAR) are a single cell clone derived from a cell line isolated from a lung tumour of KRASLSL-G12D/+; Trp53fl/fl;Rosa26A3Bi; Rag1-/- mice. Although cell line is derived from a heterozygous KRAS-G12D/WT model, the cell line is homozygous for the mutation. Mouse models of mutant KRAS-driven lung cancer with an elevated tumour mutational burden by expressing the human DNA cytosine deaminase, APOBEC3B, to mimic the mutational signature seen in human lung cancer. The ability of KRAS/KRASG12 inhibitors to cause regression of KRASG12C expressing tumours was markedly potentiated by the adaptive immune system.
Alternate name:  
CRISPR: No
Production details: Single cell clone from a cell line isolated from KrasLSL-G12D/+; Trp53fl/fl; Rosa26A3Bi;Rag1–/– mouse (KPAR) lung tumours
Model description: Single cell clone from a cell line isolated from KrasLSL-G12D/+; Trp53fl/fl; Rosa26A3Bi;Rag1–/– mouse (KPAR) lung tumours
Products or characteristics of interest:  
Cat. #: 161803
Cancer types: Lung cancer
Research Fields: Cancer
Organism: Mouse
Tissue: Lung
Model: Mutant
Primary citation: Boumelha et al. 2022. Cancer Research. 82(19):3435-3448. PMID: 35930804.
Initial handling information:  
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM media supported with 10% FBS, glutamine and penicillin/streptomycin
Subculture routine:  
Temperature:  
Atmosphere:  
Storage medium:  
Recommended controls:  
Biosafety level: 1
References:

Boumelha et al. 2022. Cancer Research. 82(19):3435-3448. PMID: 35930804.

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