The K38 Keratinocyte Cell Line has been established from the skin of neonatal WT mice. The prospective commercial application of the line is in the generation of in vitro epidermal ‘skin equivalents’ to be used as screening tools to look at the activity of NCE’s and/or NBE’s of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments Murine keratinocyte stem cell line
Production details:
Keratinocytes were isolated from neonatal wild-type BALB/c mouse skin. The cell line was established by growing cells for the first 8 passages in co-culture with 3T3-J2 fibroblast feeder cells. From passage 9 onwards cells were grown without feeder cells.
Cellosaurus ID:
CVCL_W141
Disease:
Dermatology
Cat. #:
151682
Unit size:
1×10^6 cells / vial
Research Fields:
Drug development
Organism:
Mouse
Model:
Primary line
Growth properties:
Adherent
Format:
Frozen
Shipping conditions:
Dry ice
Growth medium:
The cell line grown is low calcium FAD medium (FAD: DMEM/HAM’s F12, 3.5 : 1.1 with 0.05 mM Ca2+), with 10% FCS and several supplements at 32°C, 5% CO2. The supplements are: 0.18 mM adenine, 0.5 ug/ml hydrocortisone, 5 ug/ml insulin,10 -10 M cholera toxin, 10 ng/ml EGF, 2 mM glutamine, 1 mM pyruvate
References:
Reichelt and Haase, 2010. Methods Mol Biol. 585:59-69. PMID: 19907996 HÄ?her T et al., 2011. Stem Cell Rev. PMID: 21874280 Vollmers A et al., 2011. PMID: 21892602
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