Cell line used to study how the absence of the Zip6 transporter affects lymphocyte physiology
| Inventor | Institute |
|---|---|
| Rub?n Vicente | Universitat Pompeu Fabra |
| SKU: | 161686 |
|---|---|
| Product description: | Cell line used to study how the absence of the Zip6 transporter affects lymphocyte physiology. Zip6 Knockout (KO) line, Zip6 is strongly upregulated early during activiation of T lymphocytes stimulated using anti-CD3/anti-CD28 |
| CRISPR: | Yes |
| Production details: | Cells were maintained in RPMI 1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin. Where indicated, FBS was incubated according to the manufacturerâs instructions with Chelex 100 resin (Bio-Rad Laboratories) to generate Zn2+-free growth medium. Various amounts of ZnSO4 were added to the final medium to generate specific Zn2+ concentration conditions. Cells were grown at 37°C in a humidified 5% CO2 atmosphere. |
| Parental cell line: | Jurkat |
| Model description: | CRISPR/Cas9âexpressing Jurkat cell line used to generate a Zip6KO Jurkat cell line |
| Cat. #: | 161686 |
|---|---|
| Cancer types: | Blood cancer |
| Research Fields: | Cancer; Immunology |
| Organism: | Human |
| Tissue: | PBMC |
| Model: | Zip6KO |
| Growth properties: | Impaired Activation |
| Primary citation: | Colomar-Carando et al. 2019. J Immunol. 202(2):441-450. PMID: 30552163. |
| Shipping conditions: | Dry Ice |
|---|---|
| Growth medium: | RPMI 1640 medium |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| References: |
Colomar-Carando et al. 2019. J Immunol. 202(2):441-450. PMID: 30552163. |
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