| Inventor | Institute |
|---|---|
| Sarah A Martin | Queen Mary University of London |
| SKU: | 158349 |
|---|---|
| Production details: | JU77 cells were incubated with 10ug/ml ADI-PEG20 in RPMI-1640 media supplemented with 2% FBS and re-treated every week. After approximately 2 months, colonies were picked and seeded in a 96-well plate with 10ug/ml ADI-PEG20. A surviving clone (JU777R) was gradually expanded and maintained in 1ug/ml ADI-PEG20. |
| Cellosaurus ID: | TBC |
| Parental cell line: | JU77 |
| Disease: | Cancer |
| Cat. #: | 158349 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Lung cancer |
| Research Fields: | Cancer |
| Organism: | Human |
| Tissue: | Lung |
| Model: | Cancer Model |
| Growth properties: | Human mesothelioma cell line resistant to pegylated arginine deiminase (ADI-PEG20). |
| Cancer Types In Detail: | Lung |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | RPMI-1640 media (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 100 U/ml penicillin, and 100ug/ml streptomycin at 37?°C/5% CO2. |
| References: |
Locke et al. 2016. Cell Rep. 16(6):1604-1613. PMID: 27452468. |
|---|
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