IVRO1 Cell Line

OVCAR4-derived carboplatin-resistant HGSC model selected in vivo following tumour response and regrowth

Contributors

Inventor Institute
Michelle Lockley Queen Mary University of London, UK.
SKU: 161758
Product description: IVRO1 was established from an OVCAR4-Luc intraperitoneal xenograft in a mouse that initially responded to carboplatin before tumour regrowth, indicating development of carboplatin resistance in vivo. Tumour material was collected at necropsy and dissociated to establish the cell line. This distinguishes IVRO1 from the panel’s in vitro-selected derivatives by allowing resistance that emerged within an in vivo tumour environment to be studied after isolation. Used alongside OV4Carbo and the wider platinum-resistant panel, it therefore provides a way to compare resistance arising under different selection environments rather than examining drug exposure alone.
Gender: Female
CRISPR: No
Production details: Mice with OVCAR4 intraperitoneal xenografts initially responded to carboplatin but tumours eventually regrew indicating the in vivo development of carboplatin-resistance. Murine tumours were harvested at necropsy and dissociated to create IVR01.
Parental cell line: OVCAR4-Luc cell lines
Model description: Platinum-resistant HGSC in vivo cell line
Related tools: 161760 ; 161761 ; 161762 ; 161763 ; 161759
Cat. #: 161758
Cancer types: Ovarian cancer
Research Fields: Cancer
Organism: Human
Tissue: Ovary
Gender: Female
Model: Mutant
Growth properties: Adherent
Primary citation: J I Hoare et al. 2022. Gynecol Oncol. 167(1):96-106. PMID: 35918200.
Initial handling information: Cells should be cultured without drug but used at low passage to ensure drug resistance is maintained
Growth medium: DMEM containing 10% FBS and 1% penicillin/streptomycin. Cells should be cultured without drug but used at low passage to ensure drug resistance is maintained.
Temperature: 37° C
Mycoplasma free: Yes
References:

J I Hoare et al. 2022. Gynecol Oncol. 167(1):96-106. PMID: 35918200.

Images

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IVR01 (purple) and IVR02 (pink). The IP OVCAR4-Luc tumours of two carboplatin-treated mice were harvested at end point

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161763 OvsahoCis Cell Line Key Info View Tool

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