iPSC healthy control for neuronal differentiation (F002.1A.13) cell line

Contributors

Inventor Institute
Maria Carmo-Fonseca Instituto de Medicina Molecular Jo?„ƒo Lobo Antunes
SKU: 160738
Product description: Healthy control â?‚€?‚“ optimized for cerebellar neurons differentiation. Generation of electrophysiologically active GABAergic and glutamatergic cerebellar neurons in long-term cultures without co-culturing with other cell types. Relevant model for drug screening and for study specific pathways involved with the development of cerebellar diseases such as ataxias, a group of disorders that affect many children and adults worldwide.
Conditional: No
Production details: Reprogramming of human induced pluripotent stem cells from a skin biopsy of a female healthy donor.
Parental cell line: Human fibroblasts
Disease: Cerebellar diseases
Cat. #: 160738
Unit size: 1×10^6 cells / vial
Research Fields: Genetics;Neurobiology;Stem cell biology
Organism: Human
Tissue: Fibroblast
Model: Stem Cells
Growth properties: Healthy control
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTM1 Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3â?‚€?‚“4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 â?‚—ĚšC in a humidified 5% CO2 incubator.

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