iPSC control for BRCA1-associated cancer model cell line (2040)

Control for BRCA1-associated cancer model.

Contributors

Inventor Institute
Maria Carmo-Fonseca ; Sim?„ƒo Teixeira da Rocha Instituto de Medicina Molecular Jo?„ƒo Lobo Antunes
SKU: 160741
Product description: Control for BRCA1-associated cancer model. Investigation of tissue-specific stem/progenitor cells that represent the cells of origin of BRCA1-associated tumours, modelling early phases of malignant transformation and development of drug screening strategies aiming at preventing BRCA1 mutant hereditary cancers.
Alternate name: iPSCs control 24 â?‚€?‚“ clones 6 and 8 (IBBISTi2-A and IBBISTi2-B)
Conditional: No
Production details: Reprogramming of human induced pluripotent stem cells from skin biopsy of a familiar female healthy donor.
Parental cell line: Human fibroblasts
Disease: Hereditary breast cancer
Cat. #: 160741
Unit size: 1×10^6 cells / vial
Research Fields: Cancer; Genetics; Stem cell biology
Organism: Human
Tissue: Fibroblast
Model: Cancer Model
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTMPlus Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3â?‚€?‚“4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 â?‚—ĚšC in a humidified 5% CO2 incubator
Recommended controls: Yes, this cell line can be used as a familiar healthy control for BRCA1 c.3612delA iPSC line (2042).

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