Immortalized Fibroblastic Reticular cell line
Contributors
| Inventor | Institute |
|---|---|
| Akira Ono | University of Michigan |
| SKU: | 156505 |
|---|---|
| Product description: | Human lymphatic fibroblasts were immortalized by transfecting them with a vector that expresses HPV16 E6 and E7 genes. Primary fibroblastic reticular cells (FRCs) are stromal cells found in secondary lymphoid organs, which are major sites for HIV-1 infection of CD4+ T cells. Using the immortalized FRCs it was shown that FRCs capture HIV-1 particles and facilitate infection of T cells when FRCs contact T cells, increasing HIV-1 spread via trans-infection. |
| Production details: | Immortalized Human lymphatic fibroblasts were generated by transfection of cells with vector pLXSN16E6E7, which encodes HPV E6 and E7, and subsequent selection in fibroblast growth supplement containing G418. A clone was isolated from G418-resistant cells by limiting dilution and cultured in fibroblast growth supplement without G418. |
| Parental cell line: | Human lymphatic fibroblasts |
| Cat. #: | 156505 |
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| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Immunology |
| Organism: | Human |
| Tissue: | Lymph Node |
| Model: | Immortalised Line |
| Format: | Frozen |
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| Shipping conditions: | Dry ice |
| Growth medium: | Fibroblast Medium (FM, 2% FBS) — ScienCell, Cat# 2301 Note: The term “FM-2” used in the original publication refers to Fibroblast medium containing 2% FBS (ScienCell Cat# 2301) |
| Subculture routine: | Seed cells at 80000/ ml or 16000/cm2. Split every 2-3 days, maintain for up to two months post-revival of frozen cells. Please also see detailed protocol within the Product Datasheet in the Documentation section below. |
| Temperature: | 37°C |
| Atmosphere: | 5% CO2 |
| References: |
Murakami et al. 2018. Nat Commun. 22:9(1):2436. PMID: 29934525 |
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