An immortalised human melanocyte model used to study melanocyte biology, pigmentation pathways, and therapeutic target discovery. Save 30% on this cell line this summer. Use code SUM26CELL at checkout.
| Inventor | Institute |
|---|---|
| Pranab K Das | Amsterdam University |
| SKU: | 154099 |
|---|---|
| Product description: | Immortalized human melanocyte cell line established from neonatal foreskin by introduction of retroviral construct carrying a geneticin resistance gene and containing HPV16E6E7 open reading frames. |
| Gender: | Male |
| Conditional: | No |
| Production details: | Melanocyte cultures were established from neonatal foreskin using standard methods. Melanocytes from passage 12 were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In addition the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml. |
| Cellosaurus ID: | CVCL_S410 |
| Parental cell line: | Human foreskin |
| Cat. #: | 154099 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Skin cancer |
| Research Fields: | Cancer;Cell biology |
| Organism: | Human |
| Tissue: | Neonatal foreskin |
| Gender: | Male |
| Model: | Immortalised Line |
| Growth properties: | Cells were routinely passaged 1:4 at confluency |
| Primary citation: | Le Poole et al. 1997. In Vitro Cell Dev Biol Anim. 33(1):42-9. PMID: 9028834 |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | Ham’s F10 medium supplemented with 10ng/ml tetradecanoly phorbol 13-acetate (TPA), 0.1mM 3-isobutyl-methyl-xanthine (IBMX), 1% vol/vol Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100 ug/ml streptomycin |
| Subculture routine: | Cells were routinely passaged 1:4 at confluency |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Ivanova et al. 2008. In Vitro Cell Dev Biol Anim. 44(8-9):385-95. PMID: 18594937 Le Poole et al. 1997. In Vitro Cell Dev Biol Anim. 33(1):42-9. PMID: 9028834 |
|---|
Please note we may take up to three days to respond to your enquiry.
CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.