Immortalised Human Fibroblast Cell Line
Contributors
| Inventor |
|---|
| Pranab K Das |
| SKU: | 154112 |
|---|---|
| Product description: | Immortalised human fibroblasts are widely employed as a model system for cell and molecular biology studies |
| Production details: | Fibroblast cultures were establised from neonatal foreskin using standard methods. Fibroblasts were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In additon the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml. |
| Parental cell line: | Human foreskin |
| Cat. #: | 154112 |
|---|---|
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Cell biology |
| Organism: | Human |
| Tissue: | Fibroblast |
| Model: | Immortalised Line |
| Growth properties: | Cells were routinely passaged 1:4 at confluency |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM:M199 (1:4) supplemented with 10% FBS |
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