Immorta-MAIT CD4+ T cell clone JK-A8 cell line

Contributors

Inventor Institute
Marielle Gold ; Erin Meerseier ; Irina Kurtz ; David Lewinsohn Oregon Health & Science University (OHSU)
SKU: 159696
Product description: Mucosal-associated invarient T (MAIT) cells are innate-like T cells (a subset of T cells) that are found in blood, liver, lungs, and mucosa and are known to play a role in defense against bacterial and viral infections. MAITs have also been shown to potentially play a role in autoimmune diseases such as multiple sclerosis, rheumatiod arthritis, and systemic lupis erythematosus.
Conditional: No
Production details: Generated by single cell isolation of MR1/5-OP-RU tetramer+ cells from a human thymus. Single cells were rapidly expanded into a T cell clone using antibody to CD3 (clone OKT3) and IL-2.
Disease: Mycobacterium tuberculosis
Cat. #: 159696
Unit size: 1×10^6 cells / vial
Research Fields: Immunology
Organism: Human
Model: Immortalised Line
Growth properties: TRAV1-2 is expressed uniformly (determined by flow cytometry staining). This T cell clone binds the MR1/5-OP-RU tetramer but not MR1/6FP tetramer (negative control). Clone is MR1-restricted in its production of IFN-gamma by ELISPOT test, determined by its response to M. smegmatis-infected A549 cell line but not a M. smegmatis-infected MR1– A549 cell line.
Format: Frozen
Shipping conditions: Dry ice
Growth medium: These cell lines are not suitable for large-scale expansion following standard cell culture protocols. Following the recommended guidelines the cells should double after a week, after which cells are stable and useable for weeks. Upon receipt spin cells and resuspend them in 15ml of fresh media and add to T12.5 flask. Recommended media is RPMI supplemented with 10% FBS, L-Glut, gentamicin and 0.5ng/mL IL2. Incubate at 37 °C. Aspirate half the media and replace with fresh media every 3-4 weeks.

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