Contains full length HPV16 in episomal form
| Inventor | Institute |
|---|---|
| Aloysius Klingelhutz | The University of Iowa |
| SKU: | 154463 |
|---|---|
| Product description: | Contains full length HPV16 in episomal form |
| Conditional: | No |
| Production details: | The 7905 base pair (bp) clone pEFHPV-16W12E (gift from Dr. Paul F. Lambert, University of Wisconsin Medical School, Madison, WI) derived from an HPV-positive patient was utilized as the HPV-16 genome for our replication assays (Flores et al., 1999). One hundred micrograms of HPV-16 genomes was digested overnight in a 750- l reaction from the pUC19 vector using the restriction enzyme BamHI followed by heat inactivation of the enzyme. The entire digested DNA was then re-ligated in a large volum… |
| Parental cell line: | Human Cervical Keratinocytes |
| Disease: | Cancer |
| Cat. #: | 154463 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Cancer |
| Organism: | Human |
| Tissue: | Cervix |
| Model: | Immortalised Line |
| Growth properties: | The HPV-16-containing clones became immortal without a crisis and, at later passage, exhibited elevated levels of telomerase and higher levels of hTERT without any apparent increase in HPV-16 copy number, E6 transcript levels, or ability to degrade p53. |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | G418 selection (Have with and without feeders) |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Sprague et al. 2002. Virology. 301(2):247-54. PMID: 12359427. |
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