HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line

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The human histone H2B gene was fused to the gene encoding mCherry and Alpha Tubulin was similarly fused to EGFP.

Contributors

Inventor Institute
Francis Barr University of Liverpool
SKU: 152987
Product description: The human histone H2B gene was fused to the gene encoding mCherry and Alpha Tubulin was similarly fused to EGFP.
Parental cell line: HeLa S3
Cat. #: 152987
Provenance and Ownership: The HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line is owned by Cancer Research UK. CancerTools supplies the HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology;Genetics
Organism: Human
Tissue: Cervix
Model: Reporter
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM, 10% FBS, 5% CO2, 37?‚°C. Antibiotic selection for GFP and mCherry expression: 1µg/mL Puromycin, 4µg/mL Blasticidine, expression is quite stable but selecting at least every two passages is recommended.
Recommended controls: HeLa parental line
References:

Dunsch et al. 2012. J Cell Biol. 198(6):1039-54. PMID: 22965910.

Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation.

Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.

Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.

Bastos et al. 2010. J Cell Biol. 191(4):751-60. PMID: 21079244.

Plk1 negatively regulates Cep55 recruitment …

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