HeLa FRT/TO (YFP-Astrin 4A siRNA res) cell line

Contributors

Inventor Institute
Duccio Conti ; Viji M. Draviam Queen Mary University of London
Cat. #: 157864
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Cell biology;Cell signaling and signal transduction
Organism: Human
Tissue: Cervix
Model: Cancer Model
Alternate name: HeLa FRT/TO YFP-Astrin WT cell line conditionally expressing siRNA-resistant YFP-Astrin WT.
Production details: HeLa FRT/TO YFP-Astrin 4A cell line conditionally expressing siRNA-resistant YFP-Astrin 4A. Created by transfection of HeLa Flp recombinase cell line with pCDNA5-FRT/TO-YFP-Astrin 4A expression plasmid, followed by a brief Hygromycin selection and sorting for YFP positive using FACS.
Additional notes: siRNA oligos to target Astrin mRNA 5 UTR (GACUUGGUCUGAGACGUGAtt) or Astrin 52 oligo (UCCCGACAACUCACAGAGAAAUU). Astrin-4A mutant (RVMF to AAAA substitution) was generated using site directed mutagenesis.
Parental cell line: HeLa cell line
Format: Frozen
Shipping conditions: Dry ice
References:

31808746

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