To identify genes whose expression is controlled by YBX3
Contributors
Inventor
Institute
Matthias Hentze
European Molecular Biology Laboratory (EMBL)
SKU:
160839
Product description:
To identify genes whose expression is controlled by YBX3
Production details:
Adherent HeLa cells were grown at 37ÄÂ?°C and 5% CO2 in DMEM, supplemented with 10% FBS Gold (PAA), 1% Penicillin/Streptomycin (Sigma) and 1% L-glutamine (GIBCO). Cells were grown until 70%ÄË?Â?Â80% confluent, trypsinized, counted (Biorad TC20) and 200,000 cells were seeded per well in a 6-well dish for reverse transfection of siRNAs following the manufacturerÄË?Â?Âs recommendations (Lipofectamine RNAiMax, Invitrogen, cat# 137780755). YBX3 and control siRNAs were obtained from…
Parental cell line:
HeLa FLP-IN T-REx
Cat. #:
160839
Unit size:
1×10^6 cells / vial
Research Fields:
Metabolism
Model:
Knock-Out
Format:
Frozen
Shipping conditions:
Dry Ice
Growth medium:
Adherent HeLa cells were grown at 37?°C and 5% CO2 in DMEM, supplemented with 10% FBS Gold (PAA), 1% Penicillin/Streptomycin (Sigma) and 1% L-glutamine (GIBCO) Grown in medium containing blasticidine (5mg/ml) Cells were grown until 70%?Â80% confluent, trypsinized, counted (Biorad TC20) and 200,000 cells were seeded per well in a 6-well dish for reverse transfection of siRNAs following the manufacturer?Âs recommendations (Lipofectamine RNAiMax, Invitrogen, cat# 137780755). YBX3 and con…
References:
The RNA-Binding Protein YBX3 Controls Amino Acid Levels by Regulating SLC mRNA Abundance
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