HEK-ADAM10 CRISPR cell line

ADAM10 is a <U+0091>molecular scissor<U+0092> that proteolytically cleaves the extracellular region from over 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors and chemokines.

Contributors

Inventor Institute
Michael Tomlinson University of Birmingham
Cat. #: 157701
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Neurobiology
Organism: Human
Tissue: Kidney
Alternate name: A Disintegrin And Metalloproteinase domain-containing protein 1, CRISPR-Cas9
Product description: A disintegrin and metalloprotease 10 (ADAM10) is essential for embryonic development and impacts on diseases such as cancer, Alzheimer?‚Â’s and inflammatory diseases. ADAM10 is a ?‚‘molecular scissor?‚Â’ that proteolytically cleaves the extracellular region from over 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors and chemokines.CRISPR edited ADAM 10 HEK-293T cells.Generation of CRISPR/Cas9-knockout cell line:A guide RNA sequences was selected for human ADAM10, and the following primer pairs were used to encode these sequences: ADAM10 guide 2 (5?‚Â’-CACCGATACCTCTCATATTTACAC-3?‚Â’ and 5?‚Â’-AAACGTGTAAATATGAGAGGTATC-3?‚Â’)
Parental cell line: HEK 293T
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here.This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 …
References:

Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex

Brummer et al. 2018. FASEB J. 32(7):3560-3573. PMID: 29430990.

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