HCT 116 BRCA2 -/- 42 Cell Line

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A human BRCA2-knockout colorectal cancer model used to study homologous recombination deficiency, DNA damage repair, and PARP inhibitor sensitivity. Exclusive to CancerTools. Save 30% on this cell line this summer. Use code SUM26CELL at checkout.

Contributors

Inventor Institute
Carlos Caldas Cancer Research UK Cambridge Institute
SKU: 152628
Product description: BRAC2 inherited mutations predispose carriers to various early onset cancers, including breast and ovarian. HCT116 BRCA2 -/- human colorectal carcinoma cell line was generated to study the role of BRCA2 in DNA repair, investigate the effects of BRCA2 mutations in a range of cancers, and identify additional functions of the BRCA2 gene. This homozygous knockout cell line shows phenotypes consistent with previous reports, including loss of Rad51 foci in the presence of double-strand breaks, chromosomal rearrangements and elevated sensitivity to the DNA-damaging agents phleomycin and Parp1 inhibitors
Alternate name: HCT 116 BRCA2(-/-) clone 42; HCT 116 BRCA2(-/-)
CRISPR: No
Conditional: No
Production details: Targeted disruption of the human BRCA2 locus in HCT116 cells by homologous recombination. The gene targeting construct was generated by using a recombinant adeno-associated virus (rAAV) system. The deletion was confirmed by Southern Blot and Western Blot using antibodies against the C-terminus of the BRCA2 protein. This cell line was noted as clone 42 by the originating laboratory
Additional notes: Clone 42 was generated at the same time as clone 46 cited in Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256. The originating laboratory have advised they observed no difference in culture phenotype between clone 42 and clone 46.
Cellosaurus ID: CVCL_AS10
Parental cell line: HCT 116 cell line
Model description: Targeted disruption of human BRCA2 by homologous recombination
Disease: Cancer
Cat. #: 152628
Provenance and Ownership: The HCT 116 BRCA2 -/- 42 Cell Line is owned by Cancer Research UK. CancerTools supplies the HCT 116 BRCA2 -/- 42 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing HCT 116 BRCA2 -/- 42 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Organism: Human
Tissue: Colon
Model: Knock-Out
Cancer Types In Detail: Colon Carcinoma
Primary citation: Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: McCoy’s 5a medium modified, with 10% foetal bovine serum and 2mM L-Glutamine
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10^4 cells/cm² using 0.05% trypsin/EDTA solution. Cells are cultured at 37°C, in 95% air and 5% CO₂, and passaged every 3 to 4 days.
Recommended controls: HCT116 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256

Images

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HCT 116 BRCA2 -/- [42] Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
HCT 116 BRCA2 -/- [42] Cell Line. Late log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
HCT 116 BRCA2 -/- 42 Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

Citations And Reviews

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