HCT 116 EphA2 KO Tet-inducible Cell Line

The HCT 116 EphA2 KO Tet-inducible cell line was developed to address the involvement of EphA2 where it was shown that silencing of the protein suppressed migration and invasion.

Contributors

Inventor Institute
Sandra Van Schaeybroeck Queen's University Belfast
Cat. #: 153207
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Colorectal cancer
Research Fields: Cancer;Cell biology;Drug development
Organism: Human
Tissue: Colon
Model: Knock-Out
Growth properties: Invasion, migration
Cancer Types In Detail: Colorectal
Product description: EphA2 is an important regulator of tumour initiation, neo-vascularization and metastasis in a wide range of cancers. The HCT 116 EphA2 KO Tet-inducible cell line was developed to address the involvement of EphA2 where it was shown that silencing of the protein suppressed migration and invasion.
Conditional: Yes
Conditional description: Conditional knockdown of endogenous EphA2 expression upon treatment with Tetracycline
Production details: HCT 116 cells were transfected with pTRIPZ plasmid encoding Tet-inducible shRNA against EphA2. Stably transfected cells were selected, maintained in mediumsupplemented with 0.5ÎĚ?Ÿg/mL puromycin and induced with 2ÎĚ?Ÿg/ml doxycycline.
Cellosaurus ID: CVCL_HG05
Parental cell line: HCT 116
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
Recommended controls: HCT 116 parental line
References:

Dunne et al. 2016. Clin Cancer Res. 22(1):230-42. PMID: 26283684.

EphA2 Expression Is a Key Driver of Migration and Invasion and a Poor Prognostic Marker in Colorectal Cancer.

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