HCT 116 ADAM17 Cell Line

HCT 116 ADAM17 Cell Line showed that overexpression of ADAM-17 significantly decreases the effect of chemotherapy on tumor growth and apoptosis.

Contributors

Inventor Institute
Sandra Van Schaeybroeck Queen's University Belfast
SKU: 153209
Product description: Chemotherapy (5-fluorouracil) treatment has been shown to result in acute increases in transforming growth factor-a, amphiregulin, and heregulin ligand shedding in vitro and in vivo correlating with significantly increased ADAM-17 activity. Small interfering RNAmediated silencing and pharmacologic inhibition confirmed that ADAM-17 was the principal ADAM involved in this prosurvival response. HCT 116 ADAM17 Cell Line showed that overexpression of ADAM-17 significantly decreases the effect of chemotherapy on tumor growth and apoptosis.
Conditional: No
Production details: HCT116 cells were cotransfected with 10 ÎĚ?Ÿg of a plasmid containing hemagglutinin (HA)-tagged full-length mouse ADAM-17 (HA-ADAM-17) and a construct expressing a puromycin resistance gene. Stably transfected cells were selected and maintained in medium supplemented with 1 ÎĚ?Ÿg/mL puromycin.
Additional notes: Offered under licence from the Spanish National Research Council (CSIC)
Cellosaurus ID: CVCL_HG03
Parental cell line: HCT 116
Disease: Cancer
Cat. #: 153209
Provenance and Ownership: The HCT 116 ADAM17 Cell Line is owned by Cancer Research UK. CancerTools supplies the HCT 116 ADAM17 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing HCT 116 ADAM17 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Colorectal cancer
Research Fields: Cancer; Drug development
Organism: Human
Tissue: Colon
Model: Knock-In
Growth properties: Invasion, migration
Cancer Types In Detail: Colorectal
Format: Frozen
Shipping conditions: Dry ice
Growth medium: McCoy’s 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
Recommended controls: HCT 116 parental line
References:

Kyula et al. 2010. Clin Cancer Res. 16(13):3378-89. PMID: 20570921.

Chemotherapy-induced activation of ADAM-17: a novel mechanism of drug resistance in colorectal cancer.

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