| Inventor |
|---|
| Pranab K Das |
| SKU: | 154105 |
|---|---|
| Product description: | Halo nevus is a mole that is surrounded by a depigmented ring or ‘halo’. The formation of a halo surrounding a nevi us believed to occur when CD8+ T lymphocytes destroy the pigment producing melanocytes cells however the cause for the attack is unknown. Halo nevi are estimated to be present in approximately 1% of the general population, and are found to be more prevalent in people with vitiligo, malignant melanoma, or Turner syndrome. Primary melanocytes isolated from a halo nevus can be used as a tool for research. |
| Alternate name: | Sutton nevi, Leukoderma acquisitum centrifugum, Perinevoid vitiligo |
| Conditional: | No |
| Production details: | Autologous skin was taken from halo nevus using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10ÄÂ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100ÄÂ??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418). |
| Parental cell line: | Halo nevus |
| Disease: | Halo Nevus |
| Cat. #: | 154105 |
|---|---|
| Unit size: | 1×10^6 cells / vial |
| Organism: | Human |
| Model: | Primary line |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | HAMs F10 supplemented with 10 ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418) |
Please note we may take up to three days to respond to your enquiry.
CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.