H413 Cell Line
Exclusive toolEstablished from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a 53 year-old female patient. – H413 Cell Line
Contributors
| Inventor | Institute |
|---|---|
| Stephen Prime | University of Bristol |
| SKU: | 153425 |
|---|---|
| Product description: | Established from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a 53 year-old female patient. STNMP stage II, moderately differentiated, node negative tumour. Mutant p53, codon 68 exon 4, G to T; wild type K-, N- and Ha-ras. These cells are non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth |
| Cellosaurus ID: | CVCL_2465 |
| Disease: | Cancer |
| Cat. #: | 153425 |
|---|---|
| Provenance and Ownership: | The H413 Cell Line is owned by Cancer Research UK. CancerTools supplies the H413 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing H413 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Head and neck cancer |
| Research Fields: | Cancer |
| Tissue: | Buccal mucosa |
| Model: | Tumour line |
| Growth properties: | Adherent |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate |
| Subculture routine: | Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Suggested seeding density of 5 x 1000 cells/cm². Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability. |
| References: |
Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286. Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53. Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893. TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes. Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215. Epidermal growth f… |
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H413 Cell Line.
H413 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
