Glioblastoma WHO IV spheroid cell line, NCH705
| Inventor | Institute |
|---|---|
| Christel Herold-Mende | Heidelberg University |
| SKU: | 162330 |
|---|---|
| Product description: | NCH705 is a patient-derived IDH wildtype glioblastoma neurosphere cell line established from a primary WHO grade IV tumour resected from a 78 year old male patient. The tumour has been classified as mesenchymal subtype and assigned to the Plexus Tu methylation subclass (pediatric B). Cells grow as free floating neurospheres under serum-free conditions. |
| Production details: | Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro. |
| Cat. #: | 162330 |
|---|---|
| Cancer types: | Brain cancer |
| Organism: | Human |
| Tissue: | Brain |
| Morphology: | Grown as free-floating neurospheres under serum-free culture conditions. |
| Growth properties: | Cells grow as free-floating neurospheres under serum-free conditions in non-adherent culture vessels. Cultures are maintained at 37°C in a humidified incubator with 5% CO2. |
| Primary citation: | Alhalabi et al. 2022. Neuro Oncol. 24(1):39-51. PMID: 34232320. |
| Growth medium: | DMEM/F-12–based serum-free medium supplemented with BIT, GlutaMAX, EGF, and FGF-2. |
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| Subculture routine: | Neurospheres are passaged at high cell density by gentle mechanical dissociation. Accutase may be used if required to obtain single-cell suspensions. Cells are reseeded in fresh serum-free medium. |
| Temperature: | 37°C |
| References: |
Alhalabi et al. 2022. Neuro Oncol. 24(1):39-51. PMID: 34232320. Campos et al. 2014. J Pathol. 234(1):23-33. PMID: 24756862. |
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