Glioblastoma WHO IV NCH89 cell line

NCH89 is a patient-derived IDH wildtype glioblastoma cell line established from a primary WHO grade IV tumour obtained from a 41 year old female patient. Cells grow as adherent monolayer…

Contributors

Inventor Institute
Christel Herold-Mende Heidelberg University
SKU: 162308
Product description: NCH89 is a patient-derived IDH wildtype glioblastoma cell line established from a primary WHO grade IV tumour obtained from a 41 year old female patient. Cells grow as adherent monolayer cultures under standard conditions..
Production details: Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Cat. #: 162308
Cancer types: Brain cancer
Organism: Human
Tissue: Brain
Morphology: Adherent
Growth properties: Cells grow as adherent monolayer cultures under standard culture conditions at 37°C in a humidified incubator with 5% CO2. Medium is replaced twice per week.
Primary citation: Karcher et al. 2005. Cancer Cell Biol. 118(9):2182-2189. PMID: 16331629.
Growth medium: DMEM (high glucose) supplemented with 10% fetal calf serum (FCS), 1% GlutaMAX, and 1% penicillin/streptomycin.
Subculture routine: Cells are passaged at sub-confluent densities using 0.05% Trypsin-EDTA. After enzymatic detachment, trypsin is neutralised with serum-containing medium and cells are reseeded into fresh culture vessels. Passage number should be recorded.
Temperature: 37°C
References:

Fermi et al. 2022. Chemistry. 28(34):e202104535. PMID: 35293640.

Wirthschaft et al. 2019. Acta Neuropathol. 138(2):275-293. PMID: 31062076.

Schule et al. 2012. Scientific World Journal. 2012:697313. PMID: 22919349.

Fassl et al. 2012. Oncogene. 31(44):4698-4708. PMID: 22249262.

Campos et al. 2011. Am J Pathol. 178(5):1953-1964. PMID: 21514413.

Wentzel et al., 2010 J Med Chem 53(8):3214-26, PMID: 20329733

Konkarevic et al. 2009. Free Radic Biol Med. 46(8):1096-108. PMID: 19439228.

Tagscherer et al. 2008. Oncogene. 27(52):6646-6656. PMID: 18663354.

Siegelin et al. 2008. Mol Cancer Ther. 7(11):3566-3574. PMID: 19001439.

Kleber et al. 2008. Cancer Cell. 13(3):235-248. PMID: 18328427.

Combs et al. 2007. Int J Rad Oncol Biol Phys. 71(4):972-978. PMID: 18337022.

Karcher et al. 2005. Cancer Cell Biol. 118(9):2182-2189. PMID: 16331629.

Steiner et al. 2004. J Neurooncol. 66(1-2):129-138. PMID: 15015778.

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Glioblastoma WHO IV NCH89 cell line

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