Glioblastoma WHO IV NCH82 cell line

Glioblastoma WHO IV cell line, NCH82

Contributors

Inventor Institute
Christel Herold-Mende Heidelberg University
SKU: 162306
Product description: Glioblastoma WHO IV cell line, NCH82
Production details: Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Cat. #: 162306
Cancer types: Brain cancer
Organism: Human
Tissue: Brain
Morphology: Adherent
Growth properties: Cells grow as adherent monolayer cultures under standard culture conditions at 37°C in a humidified incubator with 5% CO2. Medium is replaced twice per week.
Primary citation: Karcher et al. 2005. Cancer Cell Biol. 118(9):2182-2189. PMID: 16331629.
Growth medium: DMEM (high glucose) supplemented with 10% fetal calf serum (FCS), 1% GlutaMAX, and 1% penicillin/streptomycin.
Subculture routine: Cells are passaged at sub-confluent densities using 0.05% Trypsin-EDTA. After enzymatic detachment, trypsin is neutralised with serum-containing medium and cells are reseeded into fresh culture vessels. Passage number should be recorded.
Temperature: 37°C
References:

Arseni et al. 2023. Cancers. 15(2):479. PMID: 36672428.

Fermi et al. 2022. Chemistry. 28(34):e202104535. PMID: 35293640.

Wirthschaft et al. 2019. Acta Neuropathol. 138(2):275-293. PMID: 31062076.

Jortzik et al. 2014. Biochimica et Biophysica Acta. 1844(8):1415-1426. PMID: 24440405.

Schule et al. 2012. Scientific World Journal. 2012:697313. PMID: 22919349.

Dokic et al. 2012. Glia. 60(11):1785-1800. PMID: 22951908.

Campos et al. 2011. Am J Pathol. 178(5):1953-1964. PMID: 21514413.

Konkarevic et al. 2009. Free Radic Biol Med. 46(8):1096-108. PMID: 19439228.

Siegelin et al. 2008. Mol Cancer Ther. 7(11):3566-3574, PMID: 19001439.

Gdynia et al. 2007. Mol Cancer Res. 5(12):1232-1240. PMID: 18171980.

Karcher et al. 2005. Cancer Cell Biol. 118(9):2182-2189. PMID: 16331629.

Steiner et al. 2004. J Neurooncol. 66(1-2):129-138. PMID: 15015778.

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Glioblastoma WHO IV NCH82 cell line

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