Cells grow as adherent monolayer cultures under standard culture conditions at 37°C in a humidified incubator with 5% CO2. Medium is replaced twice per week.
Primary citation:
Kleber et al. Cancer Cell. 13(3):235-248, PMID: 18328427.
Product description:
NCH270 is a patient-derived IDH wildtype glioblastoma cell line established from a primary WHO grade IV tumour resected from a 72 year old male patient. Cells grow as adherent monolayer cultures under standard conditions.
Production details:
Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Growth medium:
DMEM (high glucose) supplemented with 10% fetal calf serum (FCS), 1% GlutaMAX, and 1% penicillin/streptomycin.
Subculture routine:
Cells are passaged at sub-confluent densities using 0.05% Trypsin-EDTA. After enzymatic detachment, trypsin is neutralised with serum-containing medium and cells are reseeded into fresh culture vessels. Passage number should be recorded.
Temperature:
37°C
References:
Wirthschaft et al. 2019. Acta Neuropathol. 138(2):275-293. PMID: 31062076.
Kleber et al. 2008. Cancer Cell.13(3):235-248, PMID: 18328427.
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