D34 Cell Line

Exclusive tool

D34 Cell Line is derived from a leukoplakia biopsy. The cell line was notable in its proliferative capacity, considered immortal after having completed more than 100 PDs (population doublings ) when maintained on a feeder layer of irradiated 3T3 fibroblasts. D34 was categorised as having a moderate dysplasia pathology.

Contributors

Inventor Institute
Paul Harrison Cancer Research UK, Glasgow: The Beatson Institute
SKU: 152857
Product description: D34 Cell Line is derived from a leukoplakia biopsy. The cell line was notable in its proliferative capacity, considered immortal after having completed more than 100 PDs (population doublings ) when maintained on a feeder layer of irradiated 3T3 fibroblasts. D34 was categorised as having a moderate dysplasia pathology.
Conditional: No
Production details: Biopsies were trypsinized and cultured until a growing population of cells was obtained in a 9-cm plate and then passaged once to give a stock culture that was frozen. All cells were maintained on irradiated 3T3 feeders, in 10H medium. The 3T3 feeder layer was removed by treatment with 0.02% EDTA prior to RNA and protein extraction.
Cellosaurus ID: CVCL_HF98
Disease: Cancer
Related tools: D38 Cell Line
Cat. #: 152857
Provenance and Ownership: The D34 Cell Line is owned by Cancer Research UK. CancerTools supplies the D34 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing D34 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Research Fields: Cancer; Drug development
Organism: Human
Tissue: Tongue
Model: Tumour line
Cancer Types In Detail: Dysplasia;Leukoplakia
Format: Frozen
Shipping conditions: Dry ice
Growth medium: As per Cancer Res. 1997 Sep 15;57(18):3886-9. All cells were maintained on irradiated 3T3 feeders, in 10H medium (DMEM plus 10% FCS without added growth factors except hydrocortisone).
References:

McGregor et al. 2002. Cancer Res. 62(16):4757-66. PMID: 12183435.

Molecular changes associated with oral dysplasia progression and acquisition of immortality: potential for its reversal by 5-azacytidine.

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