CHO ICAM-1Fc cells secrete a dimeric human ICAM-1Fc protein. ICAM-1 (CD54) is a major ligand for leukocyte CD11/CD18 integrins and used in adhesion assays to evaluate integrin activity.
| Inventor | Institute |
|---|---|
| Nancy Hogg | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| SKU: | 152756 |
|---|---|
| Product description: | CHO ICAM-1Fc cells secrete a dimeric human ICAM-1Fc protein. ICAM-1 (CD54) is a major ligand for leukocyte CD11/CD18 integrins and used in adhesion assays to evaluate integrin activity. |
| Conditional: | No |
| Production details: | The chimeric wild-type ICAM-1Fc protein consists of the five extracellular domains of ICAM-1 joined to the hinge, CH2 and CH3 domains of IgG1. CHO cells were transfected using DEAE-dextran and the secreted proteins were collected in serum-free medium for 4 days. The proteins were purified on a Protein AÄË?Â?ÂSepharose affinity column using standard methods. |
| Cellosaurus ID: | CVCL_AS85 |
| Parental cell line: | Chinese Hamster Ovary (CHO) |
| Cat. #: | 152756 |
|---|---|
| Provenance and Ownership: | The CHO ICAM-1Fc Cell Line is owned by Cancer Research UK. CancerTools supplies the CHO ICAM-1Fc Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing CHO ICAM-1Fc Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Cell biology;Immunology |
| Organism: | Hamster |
| Tissue: | Ovary |
| Model: | Transgenic |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Recommended controls: | Chinese Hamster Ovary (CHO) |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697. Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell. Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060. A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes. Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414. LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent deta… |
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