CACO-2 cells were infected with PLCG1 shRNA lentiviral particles (Santa Cruz Biotechnology) for knocking down the gene. Selection of stable clones started 2 days after infection with 10 ?g/mL Puromycin.
Gender:
Male
CRISPR:
No
Parental cell line:
CACO-2
Disease:
Cancer
Cat. #:
161946
Cancer types:
Colorectal cancer
Research Fields:
Cancer
Organism:
Human
Tissue:
Epithelial
Gender:
Male
Model:
Knockdown
Growth properties:
A subcultivation ratio of 1:4 to 1:6 is recommended, 1 to 2 times per week.
Primary citation:
Martins, et al. 2022. Clin Cancer Res. 28(6):1203-1216. PMID: 34980600
Format:
Frozen
Storage conditions:
Liquid Nitrogen
Shipping conditions:
Dry Ice
Growth medium:
DMEM with 10% FBS, 5% CO2
Recommended controls:
shRNA scrambled control
Biosafety level:
1
References:
Martins, et al. 2022. Clin Cancer Res. 28(6):1203-1216. PMID: 34980600
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