C106 Colorectal Cell Line

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The C106 cell line was established from a 78-year old female patient with a moderately well differentiated adenocarcinoma of the rectum

Contributors

Inventor Institute
Walter Bodmer University of Oxford
SKU: 151762
Product description: The C106 cell line was established from a 78-year old female patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes’ stage A
Alternate name: C106
Gender: Female
Conditional: Yes
Cellosaurus ID: CVCL_M011
Disease: Cancer
Cat. #: 151762
Provenance and Ownership: The C106 Colorectal Cell Line is owned by Cancer Research UK. CancerTools supplies the C106 Colorectal Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing C106 Colorectal Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Cancer types: Colorectal cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Rectum
Gender: Female
Model: Tumour line
Growth properties: Adherent
Cancer Types In Detail: Adenocarcinoma
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Iscove’s Modified Dulbecco’s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine.
Subculture routine: Cells grow as small dense islands and will not form a monolayer. C106 cells grow very slowly. This cell line should be subcultured approximately every 5-8 days. On resuscitation, centrifugation of the cells (100g x 5 min) to remove DMSO improves the establishment of a viable culture. Seed cells at 4-7×10,000 cells/cm² and use 0.05% trypsin or trypsin/EDTA. Detached cells will often remain in clumps which may be further disaggregated by repeated pipetting of the cells. Following resuscitation or subculture, the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Recommended growth conditions 5% CO2; 37°C. Please also see detailed protocol within the Product Datasheet in the Documentation section below.
Temperature: 37°C
Atmosphere: 5% CO2
References:

Efstathiou et al. 1999. Proc Natl Acad Sci U S A. 96(5):2316-21. PMID: 10051639.

Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells.

Images

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C106 Colorectal Cell Line. 3 days post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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