Instituto de Medicina Molecular Jo?Âo Lobo Antunes
SKU:
160740
Product description:
BRCA1-associated cancer model. Investigation of tissue-specific stem/progenitor cells that represent the cells of origin of BRCA1-associated tumours, modelling early phases of malignant transformation and development of drug screening strategies aiming at preventing BRCA1 mutant hereditary cancers.
Alternate name:
BRCA1 c.3612delA iPSC line (242) ÄË?Â? clones 9, 1, 4 and 5 (IBBISTi1-A, IBBISTi1-B, IBBISTi1-C and IBBISTi1-D)
Conditional:
No
Production details:
Reprogramming of human induced pluripotent stem cells from a skin biopsy of a female donor heterozygous for the BRCA1 mutation c.3612delA.
Parental cell line:
Human fibroblasts
Disease:
Hereditary breast cancer
Cat. #:
160740
Unit size:
1×10^6 cells / vial
Research Fields:
Cancer;Genetics;Stem cell biology
Organism:
Human
Tissue:
Fibroblast
Model:
Cancer Model
Format:
Frozen
Shipping conditions:
Dry ice
Growth medium:
Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTMPlus Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3ÄË?Â?Â4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 ÄË?ÂÄÂC in a humidified 5% CO2 incubator.
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