Hybrid between un-transformed mouse 3T3TK3 cells and normal rat kidney cells transformed with RSV. The hybrid cells express a non-transformed phenotype. The cells exhibit anchorage-independent growth at 35C but not at 39.5C, the non-permissive temperature and showed differences in microfilament bundle formation at both temperatures.
| Inventor | Institute |
|---|---|
| Chris Marshall | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| SKU: | 153249 |
|---|---|
| Product description: | Hybrid between un-transformed mouse 3T3TK3 cells and normal rat kidney cells transformed with RSV. The hybrid cells express a non-transformed phenotype. The cells exhibit anchorage-independent growth at 35C but not at 39.5C, the non-permissive temperature and showed differences in microfilament bundle formation at both temperatures. |
| Conditional: | No |
| Additional notes: | Rat x Mouse hybrid |
| Cellosaurus ID: | CVCL_7237 |
| Parental cell line: | 3T3TK3 cells and normal rat kidney cells |
| Cat. #: | 153249 |
|---|---|
| Provenance and Ownership: | The 3.334 SC5/8 Cell Line is owned by Cancer Research UK. CancerTools supplies the 3.334 SC5/8 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing 3.334 SC5/8 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Cell biology |
| Organism: | Hybrid |
| Tissue: | Fibroblast |
| Model: | Tumour line |
| Growth properties: | Adherent |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm? using 0.25% trypsin/EDTA; 35?°C; 5% CO2.Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| References: |
Marshall et al. 1980. Exp Cell Res. 127(2):373-84. PMID: 6247175. Suppression of the transformed phenotype with retention of the viral 'src' gene in cell hybrids between Rous sarcoma virus-transformed rat cells and untransformed mouse cells. |
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