1BR3-hTERT cell line knockout for PBRM1 (clone 6)
| Inventor | Institute |
|---|---|
| Jessica Downs, Hugang Feng | The Institute of Cancer Research |
| SKU: | 161851 |
|---|---|
| Product description: | 1BR3-hTERT cell line knockout for PBRM1 (clone 6). This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis. |
| Gender: | Male |
| CRISPR: | Yes |
| Conditional: | No |
| Cellosaurus ID: | CVCL_E3VE |
| Parental cell line: | 1BR3-hTERT |
| Model description: | PBRM1 knockout |
| Products or characteristics of interest: | Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing. |
| Cat. #: | 161851 |
|---|---|
| Organism: | Human |
| Tissue: | skin |
| Gender: | Male |
| Model: | Genetically modified ; immortalised non-cancer cells |
| Morphology: | Fibroblast |
| Growth properties: | Adherent |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM + 10S + 1% Penicillin/Streptomycin |
| Temperature: | 37C |
| Atmosphere: | 5% CO2 in air |
| Biosafety level: | 1 |
| References: |
Unpublished |
|---|
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