Expresses skin cell markers; proinflammatory
| Inventor | Institute |
|---|---|
| Aloysius Klingelhutz | The University of Iowa |
| SKU: | 153752 |
|---|---|
| Product description: | Expresses skin cell markers; proinflammatory. Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies |
| Conditional: | No |
| Production details: | HPV-16 E7 expressing keratinocytes were generated by infecting normal primary cells with an LXSH retrovirus that expressed E7 and conferred hygromycin resistance. After selection in hygromycin, cells were infected with an LXSN retroviral construct that expressed wild-type HPV-16 E6 and conferred G418 resistance.ÄÂ? |
| Parental cell line: | Human foreskin keratinocytes (HFKs) established from foreskin tissue obtained from newborns |
| Disease: | Cancer |
| Cat. #: | 153752 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Cancer |
| Organism: | Human |
| Model: | Immortalised Line |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | Maintained in Keratinocyte-SFM (Gibco-BRL, Rockville, MD). G418 selection. |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Duffy et al. 2003. Virology. 314(1):196-205. PMID: 14517073. |
|---|
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