1.2B4 cell line

The cell line has applications in the study of pancreatic cell biology. 1.2B4 cells provide a method of producing pure insulin secreting cells when stimulated. This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes.

Contributors

Inventor Institute
Peter Flatt and Neville McClenaghan Ulster University
SKU: 161633
Product description: A hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line. 1.2B4 has been shown to be tumourigenic when transplanted into a SCID mouse host.
CRISPR: No
Additional notes: Patent: Flatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2).
Parental cell line: HuP-T3 cell line
Model description: Hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line
Disease: Diabetes
Cat. #: 161633
Tool sub type: Hybrid cell line
Research Fields: Disease (not cancer)
Organism: Human
Tissue: Pancreas
Model: Transgenic
Morphology: Epitheloid
Growth properties: Adherent
Primary citation: Nishimura N et. al. 1993. Int J Pancreatol 13: 31-41 PMID: 8454916.
Initial handling information: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 104 cells/cm� using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cm� can be expected.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: RPMI-1640 + 2mM Glutamine + 10% FCS
Temperature: 37° C
Atmosphere: 5% CO2
Mycoplasma free: Yes
References:

Guo-Parke H et al. 2012. J Endocrinol. 214(3):257-65. PMID: 22685334

Related Tools for Cell lines

Cat. # Tool Name
151579 U-2 OS Gal4-p300 Cell Line Key Info View Tool
151450 JIM3 Cell Line Key Info View Tool
151446 FLYRD18 Cell Line Key Info View Tool
151448 CMT 167 Cell Line Key Info View Tool
151488 B6.1.1 Mouse ES Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.