The cell line has applications in the study of pancreatic cell biology. 1.2B4 cells provide a method of producing pure insulin secreting cells when stimulated. This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes.
| Inventor | Institute |
|---|---|
| Peter Flatt and Neville McClenaghan | Ulster University |
| SKU: | 161633 |
|---|---|
| Product description: | A hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line. 1.2B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. |
| CRISPR: | No |
| Additional notes: | Patent: Flatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2). |
| Parental cell line: | HuP-T3 cell line |
| Model description: | Hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line |
| Disease: | Diabetes |
| Cat. #: | 161633 |
|---|---|
| Tool sub type: | Hybrid cell line |
| Research Fields: | Disease (not cancer) |
| Organism: | Human |
| Tissue: | Pancreas |
| Model: | Transgenic |
| Morphology: | Epitheloid |
| Growth properties: | Adherent |
| Primary citation: | Nishimura N et. al. 1993. Int J Pancreatol 13: 31-41 PMID: 8454916. |
| Initial handling information: | Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 104 cells/cm� using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cm� can be expected. |
|---|---|
| Format: | Frozen |
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry Ice |
| Growth medium: | RPMI-1640 + 2mM Glutamine + 10% FCS |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| Mycoplasma free: | Yes |
| References: |
Guo-Parke H et al. 2012. J Endocrinol. 214(3):257-65. PMID: 22685334 |
|---|
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