| Inventor | Institute |
|---|---|
| Demin Li ;Alison Banham | University of Oxford |
| SKU: | 158042 |
|---|---|
| Alternate name: | Anti-p53 TCRm monoclonal antibody |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Immunogen: | HLA-A*0201/p53 tetramers. To generate the tetramers, HLA-A_0201 (15 mg), b2m (12.5 mg), and peptide (5 mg) were added into 500 mL of refolding buffer and refolded for 48 hours. The refolding complex was concentrated and buffer exchanged to 10 mmol/L Tris-HCl, pH8.0, and then biotinylated with BirA protein biotin ligase. Biotinylated protein was then separated by FPLC, and HLA-A2/b2m/peptide monomers were isolated and subsequently stored at -80C. Aliquots were thawed and tetramerized with Extravidin on use. |
| Myeloma used: | NS0 |
| Cat. #: | 158042 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Target: | WT p53, Wild Type p53 |
| Host: | Mouse |
| Class: | Monoclonal |
| Format: | Liquid |
|---|---|
| Shipping conditions: | Dry ice |
| References: |
GB2016052674 Li et al. 2017. Cancer Res. 77(10):2699-2711. PMID: 28363997. |
|---|
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