Anti-Phospho MDM2 (Ser186) [2G2 2.2]

Monoclonal antibody which targets the critical ser186 residue for MDM2 function.

Contributors

Inventor Institute
David Meek University of Dundee
SKU: 151309
Product description: Monoclonal antibody which targets the critical ser186 residue for MDM2 function.
Conjugation: Unconjugated
Isotype: IgG1
Molecular weight: 90 kDa
Immunogen: Peptide QRKRHKS(p)DSIS. S(p) represent a phosphorylated serine. This peptide represents the portion of MDM2 around Serine 186
Immunogen Uniprot ID: Q00987
Myeloma used: Sp2/0-Ag14
Cat. #: 151309
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cancer;Cell biology;Genetics
Application: WB ; IP ; WB
Target: Murine Double Minute 2 protein (MDM2), Phosphorylated (Serine 186)
Reactivity: Human
Clone: 2G2 2.2
Host: Mouse
Class: Monoclonal
Target background: MDM2 is a ubiquitin ligase whose principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This promotes progression through the cell cycle and cell survival. MDM2 has been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumour suppressor function. MDM2 is over-expressed in many tumours, including soft tissue carcinomas and breast cancer. The phosphorylation of serines 166 and 186 is necessary for MDM2’s translocation into the nucleus, thereby facilitating interaction with p53. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumour and normal tissues. This antibody is directed against serine 186, which is crucial for MDM2 function.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
Shipping conditions: Dry ice
References:

Zhang et al. 2019. J Cell Physiol. 234(7):11330-11347. PMID: 30478915.

Wei et al. 2013. Oncogene. 32(9):1110-20. PMID: 22525275.

Lenalidomide promotes p53 degradation by inhibiting MDM2 auto-ubiquitination in myelodysplastic syndrome with chromosome 5q deletion.

Images

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Samples were separated by SDS PAGE and then Western blotted with either anti-MDM2 (top panel) or antibodies specific to phospho-ser166; phospho-ser186; total phospho-serine or total phospho-threonine of MDM2 as indicated (left panel). Densitometric analysis was performed on Western blots shown in left panel and the level of MDM2 phosphorylation was normalized with total MDM2 as indicated (right panel) Adapted from Wei et al. 2013. Oncogene. 32(9)

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