Anti-Phospho Endosulfine [CX2.1]

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Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase.

Contributors

Inventor Institute
Julian Gannon Cancer Research UK, London Research Institute: Clare Hall Laboratories
SKU: 153179
Product description: Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.Endosulfine is highly phosphorylated in Xenopus CSF egg extracts but de-phosphorylated when the eggs enter interphase.
Conjugation: Unconjugated
Molecular weight: 14 kDa
Immunogen: Synthetic peptide – KGQKYFD-Sp-GDYNMAK
Cat. #: 153179
Provenance and Ownership: The Anti-Phospho Endosulfine [CX2.1] is owned by Cancer Research UK. CancerTools supplies the Anti-Phospho Endosulfine [CX2.1] under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing Anti-Phospho Endosulfine [CX2.1] directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Neurobiology
Application: WB
Target: Phospho Endosulfine
Reactivity: Xenopus laevis
Clone: CX2.1
Host: Mouse
Class: Monoclonal
Target background: Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.Endosulfine is highly phosphorylated in Xenopus CSF egg extracts but de-phosphorylated when the eggs enter interphase.
Format: Liquid
Concentration: 0.9-1.1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice

Images

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Figure. Immunoblot of 1) cytostatic factor extracts (CSF) and 2) Interphase egg extract using two Anti-Phospho Endosulfine mAbs and a control Anti-Phospho-cdk2 mAb. Lane 1 - CSF extract containing highly phosphorylated endosulfine; Lane 2 - Interphase extract containing hypo - phosphorylated endosulfine

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