Anti-p53 [Pab DO-7]

Anti p53 monoclonal antibody used to analyse and detect the most mutated gene in cancer’ p53, enabling identification of transformed cell lines.

Contributors

Inventor Institute
David Lane University of Dundee
SKU: 151293
Product description: A monoclonal p53 antibody, the epitope recognised by DO-7 is in the N terminus of p53. DO-7 can detect wild-type and mutant p53. DO-7 recognises three of the p53 isoforms (p53, p53beta, p53gamma).
Conjugation: Unconjugated
Isotype: IgG2b
Molecular weight: 53 kDa
Immunogen: p53
Immunogen Uniprot ID: P04637
Cat. #: 151293
Tool sub type: Primary antibody
Unit size: 100 ug
Cancer types: Other
Research Fields: Apoptosis and autophagy; Cancer; Cell biology; Genetics
Application: ELISA ; FACS ; IHC ; IF ; WB
Target: p53 (N terminus)
Reactivity: Human
Clone: Pab DO-7
Host: Mouse
Class: Monoclonal
Primary citation: Vojtesek et al. 1992. J Immunol Methods. 151(1-2):237-44. PMID: 1378473
Target background: p53 is a crucial tumour suppressor involved in over 50% of cancers. It acts as a stress-responsive transcription factor and plays a vital role in regulating cell cycle arrest, promoting apoptosis, maintaining genomic stability, controlling the cell cycle, and inhibiting angiogenesis. Known as the “guardian of the genome,” p53 prevents gene mutations. Mutations in the p53 gene are common in human cancers, resulting in dysfunctional proteins unable to bind to DNA. This loss of fun…
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
Shipping conditions: Dry ice
References:

Grass et al. 2019. Leuk Lymphoma. 60(4):886-893. PMID: 30457458.

Elsensohn et al. 2018. Am J Surg Pathol. 42(3):372-375. PMID: 29112020

Kanda et al. 2018. BMC Cancer. 18(1):657. PMID: 29907137.

Murnyk et al. 2017. Oncotarget. 8(28):46348-46362. PMID: 28654422.

Gavressea et al. 2017. Anticancer Res. 37(6):2947-2957. PMID: 28551632.

Ilhan et al. 2016. J Vet Diagn Invest. PMID: 27016721.

Alsafadi et al. 2016. Oncotarget. 7(11):12331-43.

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